Platypus are exposed to many of the processes that threaten river ecosystems, such as river regulation, poor water quality and riparian habitat degradation and loss. Platypus are particularly vulnerable to these threats due to their association with permanent freshwater waterways, habitat requirements for large pools or backwaters and overhanging riparian vegetation, and reliance on macroinvertebrate prey. The threats are likely to be exacerbated by climate change, with current projections indicating increased severity and frequency of droughts, bushfires, and intense rainfall events. In 2021, platypus were listed as threatened in Victoria (FFG Act 1988). This listing highlighted the need for on-ground management interventions to support platypus recovery. Our aim was to identify potential threats to platypus in Victoria and determine where and when these threats might be most prevalent, with the ultimate goal of guiding the implementation of management interventions to support platypus. Using literature and author judgement, we characterised 10 processes that potentially threaten platypus populations (now or in the future). We identified potential variables and proxies to measure each threat, thereby allowing us to quantify each threat in a form that was amendable to mapping using existing data sources. With this information, we developed a series of maps that characterise individual threats to platypus and clipped to areas where platypus are likely to occur to identify locations where platypus and identified threats are most likely to co-occur. By identifying where and when different threats occur, these maps will support recovery efforts for platypus in Victoria and guide management interventions to maximise the resilience of platypus populations to future threats.